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The implementation of patient safety goals is the compliance behavior of health workers in implementing the standards and targets set by the hospital with reference to international patient safety goals to ensure safe services for every patient. The international patient safety goals adopted by the hospital accreditation committee have six goals. Factors that influence the implementation of patient safety goals include knowledge, workload, and patient safety culture. The purpose of the study was to obtain empirical evidence of the effect of knowledge and workload on the implementation of patient safety goals mediated by patient safety culture. This is a causative quantitative analytic study with a cross-sectional research design. The sample population of this study was 140 nurses with saturated sampling technique. The primary data was collected through questionnaire distributed by Google Form to 140 respondents. The analysis was carried out using path analysis. The results showed that knowledge, workload, and patient safety culture simultaneously had a positive and significant effect on the implementation of patient safety goals. The research findings show that patient safety culture is able to mediate the implementation of patient safety goals. The workload does not have a direct influence on the implementation of patient safety goals.